Biological Safety Cabinet Class 3

Biological Safety Cabinet Class 3-An All-Inclusive Buying Guide by Sunnyda.

Biological Safety Cabinet Class 3 is a confined working place that is particularly designed to protect the sample, environment, and personnel from pathogens.

The other name of this leak-tight cabinet is the glove box as well as the isolation gloves box because the workers do experiments by wearing the long rubber gloves that are attached with its ports.

This enclosed place is a perfect fit for those specimens and samples that cannot be examined at any of the random places because the operator is vulnerable to their germs and particulates.

From assembling to setting and from setting to installation, everything about the safety cabinet is simpler and straightforward.

Heating elements, ionizers, filter/s, lights, etc., are the components that efficiently perform their duties and filter the contaminated air.

There are numerous features, benefits, and protocols of this Biological Safety Cabinet Class 3; to know them in detail, you can stick along with this guide.

To reduce your trouble of processing this glove box, this guide has covered every possible information, so you may be able to conveniently proceed, maintain, and use your Biological Safety Cabinet Class 3.

Safety Protocol of Biological Safety Cabinet Class 3

What is a Biological Safety Cabinet Class 3?

Biological Safety Cabinet Class 3 is completely enclosed and leak-tight, but regardless of being leak and gas-tight, it helps in ventilation, so air can easily enter in and discharge out from this cabinet.

The contaminated air from the outside environment does not directly enter inside the gas-tight Class III BSC; first, it passes through the HEPA filter, and after getting crystal clean, it goes inside the cabinet, so the samples that are under inspection stay safe.

In the same way, when the air leaves the cabinet, it is also filtered by two HEPA filters.

In simpler words, only filtered and pure air enters inside the cabinet, and when air discharges out of the cabinet, it is also filtered and pure because it has passed from the two HEPA filters.

Though all its components are crucial; however, the HEPA filter is most functional and facilitating due to its act of filtration.

Due to the exceptional filtration system of this unit, you can conveniently examine and culture your desired sample. So, instead of getting worried about the atmospheric exposure of high-risk biohazards, you can conveniently deal with your infectious elements, products, and materials.

Additionally, you can also study toxins and infectious agents that can cause life-threatening diseases or infections if you will study them somewhere else.

Despite the availability of standardized-sized biosafety cabinet class 3, many users prefer custom-built, according to their desire and the samples that are anticipated to manipulate inside it.

It is compatible with all biosafety levels: 1, 2, 3, and 4, but among these four, its features and functions are more favorable for biosafety level 4.

Biological Safety Cabinet Class 3

Why is Biological Safety Cabinet Class 3 Called a Glove Box?

If you are going to conduct experiments in Biological Safety Cabinet Class 3, wearing long-sized gloves is compulsory; that is why it is known as a glove box as well.

These arm-sized rubber gloves are designed to protect the users (who are wearing them) from contamination.

These long butyl gloves ensure complete safety as the germs, or harmful products do not touch the wearer’s skin.

These gloves are attached with the cabinet’s ports; if one pair wears out, the older ones can be easily replaced with the newer ones.

Glove box’s long and heavy-duty gloves are not autoclavable; for disinfecting them, you can use isopropyl alcohol.

Biological Safety Cabinet Class 3 Gloves

What are the Significant Features of Biological Safety Cabinet Class 3?

The most salient features of Biological Safety Cabinet Class 3 are:

• The air that is discharged out of it is filtered twice via high-quality HEPA filters; therefore, the environment and products are filtered up to 99.999%.

• It contains dual exhaust filters that work exceptionally well than the single-stage design; typically, it provides more than 100.000 times protection.

• If something goes against the casualty, the alarm system updates you; for instance, if the casual airflow of Biological Safety Cabinet Class 3 is interrupted, the microprocessor-based alarm control system alerts you for the situation.

• The Magnehelic gauge monitors the negative pressure at the work zone—the negative pressure ward off the migration of infectious materials.

• The leak-tested gauntlets are assembled inside the biosafety cabinets that guarantee the utmost protection from every irrelevant and unwanted particle.

• The pass-through, which is integrated with interlocking doors, aids in the safe materials exchange. Since you don’t have environmental contamination.

• The comfortable design of this safety cabinet is specifically designed to permit you to sit in front of it for hours and hours without getting tired.

• In addition, it also includes the pass box that has two doors and is made up of electromechanical locks – this pass box helps you transfer the objects comfortably and safely.

• Three HEPA filters (High-Efficiency Particulate Air) make sure that the biosafety cabinets are sterile and safe for the environment as well as users.

• The large LCD is responsible for monitoring the three filters and pressure parameters.

Epoxy Coated Pass Box of Biological Safety Cabinet Class 3 

What are the Benefits of Biological Safety Cabinet Class 3?

The Biological Safety Cabinet Class 3 is equipped with hundreds of benefits, but a few of those are stated below:

Highly Efficient

The Biological Safety Cabinet Class 3 is highly efficient because, unlike other safety chambers, it doesn’t have one filter but three.

These three HEPA filters ensure the ultimate safety of not only the environment where the experiments are being conducted but it is also designed for creating the protective barrier between the harmful products and workers.

Hurdle-Free Usage

The biosafety cabinet class 3 is not only protective, but it is also easy to use. The user-friendly structure fully supports the laboratory technician, so they can maneuver their sample all alone.

From loading to disinfection and unloading can be done by only one person, and no additional personnel is required.

Comfortable and Ergonomic Design

The well-aligned design uplifts the flexibility of the experiments and researches; for instance, the sealed yet ventilated design helps in conducting the experiments of materials that pose to bring hazardous materials and harmful chemicals along with them.

Furthermore, the pass box of this biosafety unit also ensures the safe transfer of the sample into and out of the workplace.

The pass box is a double door and has electromechanical locks; these two doors also aid in the more effortless movement of the items from and into the cabinet.

Besides the airtight design and pass box, the exhaust system also maintains the airflow; the exhaust system is also responsible for ensuring the negative pressure (at least 120 pa) inside the unit.

Ease in Maintenance

It is robust and impermeable in quality, so it neither wears out soon nor gets dirty; you can clean it with any cleaner or solvent.

But at the time of the cleaning, you need to be extra vigilant, so the HEPA filters remain safe and dry. If they get damped, keep the unit turned off until it gets dried.

Innovative and Latest

The sterilized and disinfected parts of this innovative unit guarantee the safe handling of even high-risk samples.

This Biological Safety Cabinet Class 3 is launched to meet all the emerging requirements, so you become capable of using all the latest equipment and studying all the challenging samples.

The risky materials that can pose the aerosol transmission can also be manipulated easily; even the samples that don’t have therapies or vaccines can also be tested at this safe place.

The Class III Biosafety Cabinet can be installed against the microorganisms of levels 1 to 4 because it efficiently works against all the microorganisms.

Reliability and Accuracy

This ultimate cleaning and sterilizing unit create a barrier between the samples that are being researched and the one who is doing the research.

The most crucial purpose of this unit is to produce outright containment and handle the deadliest pathogens and biohazards.

This long-lasting leak-tight cabinet creates 99.999% of cleanliness at the workplace, so all your experiments run successfully at this reliable place.

Less Noise and No Vibration

It is obvious that the noise and vibration take the attention off and disrupt the peaceful environment.

Therefore, to protect the users from this annoyance, this biological cabinet is designed in a way that it creates minimal noise, which is even negligible, so it couldn’t interrupt anybody.

Along with the noise, this biosafety unit is also vibration-free, so all the tools and equipment inside it remain safe and arranged at their place.

Ergonomic Design of Biological Safety Cabinet Class 3

What Factors Should you Consider Before Buying your Biological Safety Cabinet Class 3?

The moment when you are picking a Biological Safety Cabinet Class 3, you are supposed to consider the following factors as they can make your selection worthier:

Size

Before picking up one, you are supposed to consider the available space of your laboratory.

Buying it in a bigger size than the available space can cause trouble; thus, it is better to measure the space appropriately and then place your order accordingly.

Lighting Options

It includes ultraviolet light, LED light, or fluorescent bulbs. Before buying it, you should consider whether the light options are installed according to your choice or not.

A UV bulb is used to create sterility in the environment but LED light and fluorescent light illuminates the cabinet so you can manipulate your products efficiently.

You are supposed to check the number of the lights and the quality as well. You also increase or decrease the number of lights in your class 3 biological safety cabinet, according to your choice.

Energy Efficiency

Usually, Biological Safety Cabinet Class 3 consumes a lot of energy as all of its components use energy to run.

Despite this, many cabinets are still constructed by considering the importance of energy saving. Hence, you should buy a cabinet that is capable of saving energy.

For energy-saving, the latest DC ECM motor is preferable because it is 70% Energy savings as compared to AC motor.

Regardless of the voltage fluctuation, it provides a stable airflow.

Additionally, to ensure energy efficiency, the Biological Safety Cabinet Class 3 undergoes different tests; hence, you should also pass your safety cabinet through different tests before paying the money.

Price

Biological Safety Cabinet Class 3 is available at different prices, and the prices vary according to the size, material, and variations in the components.

If you reconfigure it according to your choice, the price will increase to a large extent. So, you can buy the one that fulfills your need yet doesn’t break your budget.

UV Light of Biological Safety Cabinet Class 3

What are the Applications of Biological Safety Cabinet Class 3?

You can install the Biological Safety Cabinet Class 3 at the below-mentioned sites:

• Microbiological Laboratories

• Biomedical Laboratories

• All Hazard Receipt Facilities (AHRF)

In short, a biohazard safety cabinet can be used for all laboratories and biosafety levels (1-4).

Application of Biological Safety Cabinet Class 3

What Material is Used for the Construction of Biological Safety Cabinet Class 3?

Biological Safety Cabinet Class 3 is constructed by the use of stainless steel metal; this powerful metal provides it durability and resistance.

The reason for using stainless steel is its fire-resisting property, sustainability, and aesthetic appearance; additionally, it is also easier to clean.

The front window is also made up of stainless steel, and it has 5mm transparent glass to increase visibility.

Commonly, tempered glass is used in the front window because it is safer and more resistant than regular glass.

Anti Rust Stainless Steel for Biological Safety Cabinet Class 3 

What are the Components of Biological Safety Cabinet Class 3?

All the efficient components together make the most high-quality Biological Safety Cabinet Class 3.

Ultraviolet Lamp

UV lamps make sure that the work area is effectively sterilized and disinfected; it efficiently gets rid of virucide, germicide, and DNA contamination.

Angled Cabinet

An angled front makes sure of an ergonomic design and helps you to carry out the experiments in a decent working posture.

ULPA Filter

The air that is exhausted out, filtered two times by highly efficient ULPA filters as they maintain efficiency of 99.999% for the particles that are 0.1~0.2μm.

Gloves

The cabinet includes one pair of rubber gloves; these gloves protect the human skin from the direct exposure of germs and harmful chemicals.

Fluorescent Lamp

The one-piece of a fluorescent light is also embedded inside the Biosafety cabinet; this lamp provides sufficient light to the researcher, so they can pursue their experiments efficiently.

Remote Control

The remote control avoids your direct contact with the germicide, and it’s also favorable for fending off the cross-contamination.

Through remote control, the process of maintaining the antiseptic and hygienic environment becomes easier.

Drain Valve

The one drain valve is attached under the workplace of the cabinet; it helps in keeping the Biological Safety Cabinet Class 3 decontaminated.

Pass Box

Pass box eases the safe transformation of the products in and out of the workplace – it also protects the cross-contamination.

Pressure Meter

It displays the pressure of the work zone area.

99.999% Efficient ULPA Filter of Biological Safety Cabinet Class 3

What are the Specifications of Biological Safety Cabinet Class 3?

The specification of a Biological Safety Cabinet Class 3 is explained here, but you can reconfigure the specification according to your choice and requirements.

• Internal Sizes: 1200x660x700

• External Sizes: 2105x822x1300

• Filtration: H14 HEPA/ULPA filters

• Fans: 1 Double motor fan

• Average Face Velocity: 50m/s

• Power Consumption: 500 W

• Noise Level: 58dB

• Operation Condition: Class ISO 3

• Glove Ports: 300mm glove ports

• Glove Sleeves:4mm neoprene

• Voltage-Frequency: 110-220 V / 50-60 Hz

HEPA Filter of Biological Safety Cabinet Class 3

What is the Performance of the Filtration System in a Biological Safety Cabinet Class 3?

Biological Safety Cabinet Class 3 is different from other classes because it consists of three HEPA filters positioned at different places.

Hence, the uncontaminated air enters and discharges out of the cabinet.

Air enters the cabinet by passing through the one HEPA filter, and two HEPA filters are laid in series that clean the air before it goes outside.

In a few biosafety cabinets, many additional HEPA filters are also incorporated inside the facility to perform certain special tasks; for instance, a HEPA/Carbon Filtration System or Nuclear, Biological, and Chemical (NBC) Filter Systems that protects the workplace from several unknown risks.

Filtration System in a Biological Safety Cabinet Class 3

Does Biological Safety Cabinet Class 3 Need a Ductable System to Perform?

Well, you can use the Biological Safety Cabinet Class 3 in both ways: with a ducting system or without a ducting system.

It depends upon the nature of the work that is being conducted into the class III biological safety cabinet.

A ductable system provides an additional protective layer against environment protection; this system does not let the air recirculate but keeps the environment and operators protected from the biohazards.

Stainless Steel Ducted Biological Safety Cabinet Class 3

What Parameters Should you Consider For Relocating your Biological Safety Cabinet Class 3?

When needed, you can replace your Biological Safety Cabinet Class 3 with different laboratories, but before relocating, you should ensure the following parameters:

• Check the availability of the power that a blower needs to run appropriately; if the place can provide that much power, then you can relocate it.

• Besides power, the flow rate should also be considered.

• If the duct system is being used, the suggested duct diameter is 8 inches, and it must be checked over there.

• Before moving it to the new location, it must be subjected to gaseous decontamination in advance.

• After relocating to a new site, it must be recertified.

• Risk assessment and other issues must be mitigated appropriately.

Gaseous Decontamination of Biological Safety Cabinet Class 3

Why is a Biological Safety Cabinet Class 3 Safe to Use?

The following salient features of the Biological Safety Cabinet Class 3 make it safe and secure to use; moreover, these features also assist in developing a comfortable environment for the user.

• The footrest, hand rest, and an angled front of the safety cabinet help to increase your comfort and convenience. It also helps in maintaining the correct sitting posture.

• Similarly, an ergonomic lab chair also facilitates the operator to perform consistent experiments.

• The alarm system keeps the operator safe as it sounds if there is any unrequired situation.

• For safe and straightforward monitoring, it also includes the magnehelic gauge, which is mounted at the back of the work zone and helps to monitor everything with ease.

• The antimicrobial coating also inhibits the growth of disease-causing microbes.

• The interlocking doors permit the contamination-free transfer of the materials.

• Instead of single-stage ULPA filters, it contains dual exhaust ULPA filters that offer suitable protection.

• A leak-tested and single-piece gauntlet maximizes the protection of the user and environment.

• A user-friendly control system is also available.

Magnehelic Gauge of Biological Safety Cabinet Class 3

Why is the Biological Safety Cabinet Class 3 a Premier Solution for High Containment Laboratories?

Class 3 Biological Safety Cabinet is crucial for sustaining high containment in the labs and research zones, and this containment is due to an efficient workflow maintained by the integration of analytical instruments.

Safe ingress and egress of products is also an edge for creating containment.

There are different methods (rapid transfer ports, integral autoclaves, dunk tanks) that handle the safe entry of the products, and eventually, these methods help in maintaining the uncontaminated environment.

Similarly, for fumigation/decontamination, different ways for the safe elimination of materials are also incorporated.

Integral Autoclave of Biological Safety Cabinet Class 3

What Accessories are Required While you are Working in your Biological Safety Cabinet Class 3?

Buying a Biological Safety Cabinet Class 3 with its accessories is always comfortable because accessories protect the researcher from different expected and unexpected hassles.

There are many optional accessories as well that you can skip if you have less budget or if you think they are not essential for you.

The below are some of the standard and helpful accessories:

Ultraviolet Lamp (Germicidal)

• UV lamps help in 253.7 nanometers emissions that assist in decontamination.

Ergonomic Foot Rest

• The footrest is designed for the rest of the operator’s feet, creating correct posture, and for protection from the tiresome.

• The height can be adjusted according to comfort.

• Usually, footrests have chemical-resistant finishing and anti-skid coating.

Ergonomic Lab Chair

• Often, the lab chair meets Class 100 cleanliness standards.

• Different kinds of lab chairs are available, but a preferable lab chair is made up of PVC material.

• The ergonomic and adjustable lab chair is best for providing the comfortable to the operator; the preferable height is between 395-490 mm (15.6″ to 19.3.”)

Anti-Blowback Valve

Formalin Vaporizer

Electrical Outlets and Fixtures

Ergonomic Chairs for Biological Safety Cabinet Class 3

What is the Standard Compliances of Biological Safety Cabinet Class 3?

The performance, quality, and effectiveness of Biological Safety Cabinet Class 3 are verified by different tests, certifications, or compliances standards:

• TGA, FDA, or WHO

• 2., AS2243.3., AS2243.3 (Australia)

• NSF49 (US)

• EN12469 (Europe)

• AS1807 Series (Australia)

The following are the most particular processes or the components of Biological Safety Cabinet Class 3 that undergo the tests.

If they perform according to the set standards then the cabinet is governed for sale; otherwise, up-gradation is made.

• Testing the work zone’s air velocity

• Testing of air barrier (a barrier in between the product and operator)

• Filter integrity (leak testing)

• Gas tightness

• Work zone integrity (work zone leak testing)

• Testing of illuminance in the work zone

• Effectiveness of UV light

• Sound level testing

Air Tight Work Zone of Biological Safety Cabinet Class 3

What is the Purpose of Anti-Microbial Coating of the Biological Safety Cabinet Class 3?

There is not only one component that maintains the hygienic environment inside the Biological Safety Cabinet Class 3.

Many parts (HEPA filter, UV lamp, antimicrobial coating) work together to create a barrier between infectious product and operator.

An antimicrobial coating is applied on various surfaces to kill or reduce the growth of harmful microorganisms that can cause incurable diseases.

Besides maintaining the uncontaminated environment, this coating also provides durability and resistance to corrosion.

What are the Advantages of the Alarm Monitoring System of Biological Safety Cabinet Class 3?

An alarm installed inside the Biological Safety Cabinet Class 3 keeps the user safe from every kind of unwanted and unexpected situation.

If anything alarming happens, the audio and video alarm will alert the user, so a preventive measure should be taken timely.

Typically, an alarm is attached to the sash; if an operator moves the sash in an inappropriate position, the alarm will sound up and signify the operator instantly.

There is also another kind of alarm, an airflow alarm that notifies the operator if there is any disruption in the pattern of airflow.

If the airflow alarm shows an indication of anything unusual, the operator should cease the work and immediately inform the supervisor.

Taking help from the instructional manual can also cover this aspect.

You can get your safety cabinet that has one or both of these alarms (sash alarm or airflow alarm).

Monitoring System of Biological Safety Cabinet Class 3

What is the Purpose of the Blower System in Biological Safety Cabinet Class 3?

The blower (fan) is one of the most crucial components of Biological Safety Cabinet Class 3.

It is driven by a motor and helps to draw air into the air grille and around the user – this way, the operator is completely protected.

After the air grille, the air passes underneath the work surface and then goes back to the cabinet.

The main purpose of the blower (fan) is to channel the air to the different parts of the gas-tight cabinet.

Explosion Proof Blower of Biological Safety Cabinet Class 3

Conclusion.

Biological Safety Cabinet Class 3 is a leak-tight cabinet that creates an absolute barrier between the operator and the material that is under observation, so a particulate-free environment is developed.

The user-friendly and seamless design makes it easy to use for everyone; similarly, its unparalleled features and unbeatable performance makes it a perfect option against pathogens.

You can apply it in every kind of laboratory and can govern safe experiments without risking your life.

Excellent Quality Biological Safety Cabinet Class 3